| Background & Objective |
| Materials & Methods |
| Results |
| Conclusions |
| References |
| Acknowledgements |
| Project Information |
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Materials & Methods
Plant Material
Genomic DNA from 94 Recombinant Inbred Lines (RILs) derived from a cross between Cicer arietinum (ILC72)
and Cicer reticulatum (Cr5-10) were used for genotyping. Genomic DNA from resistant (ILC 72) and susceptible
(Cr5-10) parents was used as controls.
SNP Selection and KASPar Assay Design
Eight KASPar SNP markers were designed based on existing SNP haplotype markers found within two genome
regions of QTL linkage map carrying genes for Ascochyta resistance in chickpea (Fig. 1). For this set
of eight SNPs, primers were designed for KASPar genotyping using a tool provided by KBiosciences based
on the SNP locus sequence (about 50 base pairs (bp) flanking each side of the SNP are required for the
design). The output provides sequence information for two allele-specific oligonucleotides of about 40
base pair (bp) in length and one common oligonucleotide of about 20 bp in length, all of which are
standard, unmodified and unlabelled oligonucleotides.
Fig. 1: ILC 72 X CR5-10 Linkage map [ascochyta blight resistant marker on Linkage Group (LG) 3 and 6]
[Click on the figure to view its larger version.]
