The following conclusions can be drawn based on the results of this project:

• The KASPar SNP assay using direct hybridization eliminates the need for gel electrophoresis and manual gel tracking and therefore allows accurate and high speed detection of SNPs across thousands of individuals.


• MAS generally involves the assaying of a few markers across thousands of individuals, and therefore, requires low cost/genotype, short assay time, and simplicity.


• In this study, we have developed the resources for a relatively high-throughput method of selection for the Aschochyta resistance genes on LG-3 and LG-6 by developing and validating eight KASPar SNP markers.

References