| Background | Objectives | Materials & Methods | Results | Conclusions | References | Acknowledgements | Project Information |
Project Information
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Name:
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Prakriti Rohini Pratijit |
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Grade Category:
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7-9 (Grade 7) |
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Subject Area:
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Biology (Biotechnology) |
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Project Type:
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Experimental |
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Language:
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English |
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Project Summary:
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Chickpea (Cicer arietinum) is one of the most important pulse crops in the world. The aschochyta blight caused by the fungus Aschochyta rabiei is the most damaging pathogen of the chickpea. Poor understanding of variation in this fungus has hindered the breeding for resistance to ascochyta blight in Western Canada as well as other parts of the world. The absence of durable resistance in the field has been attributed to the appearance of new pathotypes of this fungus. To understand the pathogenic and genetic variability of A. rabiei, ten isolates of A. rabiei that were collected from four different countries (Canada, Syria, Israel and USA) are used in this study. By using conventional pathogenicity tests and genome analysis with SSR (simple sequence repeat) molecular marker, we demonstrated that the SSR markers distinguish variability within and among the pathotypes of A. rabiei collected from different part of the world. No correlation between SSR marker and division of the isolates in pathogenic groups could be established. |
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Equipment Used:
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PCR machine, humidity chamber, polyacrylamide gel equipment, personal computer, digital camera, other equipment commonly found in a plant biotechnology research lab. |
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Software Used:
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Eversoft 1stPage 2000 HTML editor, Microsoft PowerPoint 2003, GIMP Image Manipulation Program v. 2.6.4, Mozilla Firefox browser. |
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Source of the Idea:
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I have developed a keen interest in biotechnology, mainly because of my mother's background in molecular plant pathology. The idea for this project came about after a meeting with my mentor Dr. Sabine Banizza. I think it is a worthwhile cause to be able to screen for pathogenecity of various strains of fungi in order to understand the interaction between the crop host and the pathogen, which will help us in developing disease-resistant crop varieties. |
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Special Skills:
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Basic HTML programming; Digital image capturing and editing. |
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Awards:
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Genome Prairie Genomics Award at the Saskatoon Regional Science Fair 2009 ($100 and a trophy)
Biotechnology Award at the Saskatoon Regional Science Fair ($100 and a certificate) |
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Past projects:
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None. Participating for the first time. |
Background | Objectives | Materials & Methods | Results | Conclusions | References | Acknowledgements | Project Information © Prakriti Rohini Pratijit. All rights reserved. |