Results for the Folin-Ciocalteau Assay.
Results for the Bacterial Susceptibility Tests.
Results for the Ames Test.
Results
The FRAP assay is a known method used to measure the total antioxidating
capacity of biological samples. It depends on the reduction of Fe (III)-TPTZ
complex to the Fe (II)-TPTZ by a reductant at a low pH level. Fe (II)-TPTZ
has an intensive blue colour and can be monitored at 593 nm wavelength
(Reference 1). Quantitation
for this assay was done by analyzing a calibration curve made from the
absorbance of iron (II) sulphate at varying concentrations (absorbance
versus mmol/L iron (II) sulphate). This curve is used to relate the
absorbance of the samples to mmol iron (II) sulphate formed .
Figure
2: Antioxidant
activity (AC) of tree bark extracts was showed as equivalent of umol Fe(II)
formed. White Spruce extract had highest AC than all other three extracts. The AC
was also greater from 100ºC extracts than from 50ºC
extracts for all four kinds of bark.
Like the Folin-Ciocalteau Assay results,
the White
Spruce bark had the greatest amounts of antioxidizing activity and the
Jack Pine bark the least. Once again, the 100ºC
extracts had a higher level of antioxidating activity compared to the 50ºC
extracts from all four kinds of tree bark.
