Material

                    It is the tension  between creativity and skepticism that has produced the stunning and unexpected findings of science.     —Carl Sagan 

Home Abstract Background Question Hypothesis Objective/variable Material Procedure Results Analysis Discussion Sources of Error Conclusion Future Research Appendix Reference Acknowledgement

 

Materials:

Device and apparatus: Chemical and bacteria: Glassware and utensil:
Autoclave

Antibiotics:
-spectinomycin

96-well plates

Shaking incubator Bacteria: E. coli (DH5α)

Erlenmeyer flask

Ultrasound machine 
-frequency: 42kHz 		LB broth

Inoculating loop
Plate reader Nutrient agar plates Petri dish 

Specphotometer (Ultraspec™)
-wavelength: 600nm

   

Bunsen burner

   
Graduated pipette  
multichannel pipette    

                 

Figure 3. Antibiotic, spectinomycin                Figure 4. Specphotometer. Used to determine the bacteria 
used in the experiment.                                 concentration in LB broth (liquid nutrient for bacteria) that 
                                                        is suitable for plate reader. 

          

Figure 5. Shaking incubator. It is used to grow bacteria         Figure 6. LB broth. The liquid nutrient 
 culture everyday, at 37 degree Celsius.                                 for the bacteria E.coli.

    

Figure 7. Ultrasound machine. Sonication was done here at         Figure 8. Plate reader, used to determine the concentration frequency of 45kHz.                                                                     of E.coli in order to generate the data. 

 

 

 

 

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Enhancement of Antibiotic Action with an Application of Ultrasound
by Liz Meng: lizmeng10@hotmail.com
Victor Feng: haovictor_feng@hotmail.com
Sir Winston Churchill High School, Calgary.AB

01/05/2007