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Project Information
Introduction
Purpose
Hypothesis
Materials
Methods
Results
Conclusions
Acknowledgements

Materials

Materials for part A: Liver Cell Isolation

  1. Glassware such as beakers, volumetric flasks, test tubes, pipettes
  2. Buffer, Cell culture Media
  3. Digestive enzymes
  4. Surgical instruments and materials such as scissors, hemostats, tweezers, gauze, catheter
  5. Filter paper
  6. Centrifuge and centrifuge tubes
  7. Water bath heated to 37°C

Materials for part B: Dosing of Liver Cells with NDGA
  1. Nordihydroguaiaretic acid (NDGA)
  2. 96-well microplates
  3. Cell culture media
  4. Pipettors and pipette tips
  5. Water bath heated to 37°C
  6. Trypan Blue
  7. Hematocytometer
  8. Bright Field Inverted Tissue Culture Microscope

Materials for part C: Investigation into a Potential Mechanism by which NDGA Causes Liver Cell Death
  1. NDGA
  2. Glutathione
  3. HPLC system and HPLC column
  4. UV-Vis Spectrophotometer
  5. Pipettors and Pipette tips
  6. Shaking water bath heated to 37°C
  7. Glassware such as beakers, volumetric flasks, pipettes, test tubes
  8. Centrifuge and Ultracentrifuge
  9. pH meter
  10. Buffer and other necessary chemicals (i.e. NADPH, acetonitrile, MgCl2)
  11. Liver cell microsomes

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.: (C) Mithum Kularatne and Andrew Plishka - Canada Wide Virtual Science Fair 2007 :.