One Shade Too Dark

--- tale of condensed tannins in faba bean seed coats　

RAPD stands for random amplification of polymorphic DNA. It is a type of PCR reaction, but the segments of DNA that are amplified are random. The scientist performing RAPD creates several arbitrary, short primers, then proceeds with the PCR using a large template of genomic DNA, hoping that fragments will amplify. By resolving the resulting patterns, a semi-unique profile can be gleaned from a RAPD reaction. (taken from http://en.wikipedia.org/wiki/RAPD)

Primer Name   Source                      Primer Sequence (5'-->3')

A-01                   OPERON                   CAGGCCCTTC

A-04                   OPERON                   AATCGGGCTG

A-07                   OPERON                   GAAACGGGTG

A-11                   OPERON                   CAATCGCCGT

A-15                   OPERON                   TTCCGAACCC

B-01                   OPERON                   GTTTCGCTCC

B-04                   OPERON                   GGACTGGAGT

B-07                   OPERON                   GGTGACGCAG

B-11                   OPERON                   GTAGACCCGT

B-15                   OPERON                   GGAGGGTGTT

C-01                   OPERON                   TTCGAGCCAG

C-04                   OPERON                   CCGCATCTAC

C-07                   OPERON                   GTCCCGACGA

C-11                   OPERON                   AAAGCTGCGG

C-15                   OPERON                   GACGGATCAG

D-01                   OPERON                   ACCGCGAAGG

D-07                   OPERON                   TTGGCACGGG

D-11                   OPERON                   AGCGCCATTG

D-15                   OPERON                   CATCCGTGCT