Engineering a Cure for Diabetes Results
Transcription of IL-13 and CTB-IL13
Translation/ Glycosylation of IL-13 and CTB-IL-13
In Vitro Bioassay
Results: In Vitro Bioassay
In vitro bioassays were conducted to determine if the plant-produced recombinant proteins could induce T-cell proliferation. Soluble protein extracts were directly used for the assay. As shown in Fig. 7, both plant-derived IL-13 and CTB-IL-13 effectively induced T-cell proliferation. As expected, the protein extracted from empty vector-transformed plants showed no activity. These data suggest that the plant-derived IL-13 and CTB-IL-13 act like the mammalian-produced IL-13 cytokine and retain its biological and functional authenticity.
