Western blot was performed to detect transgenic plant-produced recombinant proteins. Anti-IL-13 polyclonal antibodies identified 4 proteins only present in pDW7- or pDW6-derived transgenic plants (Fig. 6A). This is quite interesting as only one protein is expected (one gene-one protein theory). The reasoning behind this is believed to lie in post-translational modifications (glycosylation). Thus, a deglycosylation assay was conducted using N-glycosidase F (Fig. 6B). After treatment, the amount of larger proteins decreased while the amount of the smaller proteins increased, suggesting the large recombinant proteins were N-glycosylated in transgenic plants. Similar glycosylation was found in human cells in previous studies.