Method
IV) Gas-Sampling Procedure (N2O)
Materials
-transparent plastic tarp
-1m plastic tubing
-16 syringes
-32 two-way stop cocks
-needle
-duct tape
-timer
-5m of wire
1) Ensure that each model is airtight by covering them with a plastic tarp cover lined with heavy-gage wire and secure to container edges with duct tape.
2) Place an opaque garbage bag overtop of each model in order to prevent photosynthesis.
3) Take one ambient air sample from the middle of the room by pumping a syringe several times.
4) Place the piece of tubing connected to the pump inside one of the holes drilled in the tarp piece. Place another piece of tubing inside the second hole, and attach a syringe.
5) Pump the syringe attached to the tubing of each model immediately after covering for a 0 minute gas sample. Remove the syringe and immediately replace with another.
6) Remove stop cock from syringe and replace with needle. Using the needle, insert the gas sample into a labelled vacutainer.
7) Repeat for each of the models. Take a final sample from each after two hours, and insert each into labelled vacutainers.
Syringe and needle |