Managing Greenhouse Gas Exchange in a Constructed Wetland Model:
Part 2: The Effects of Peat, Water Content, and NH4NO3 on CO2, CH4, and N2O Emissions
Method
II) Gas-Sampling Procedure (CO2 and CH4)
Materials:
-transparent plastic tarp
-1m plastic tubing
-16 syringes
-duct tape
-timer
1) Make a hole in each tarp piece.
2) Ensure that each model is airtight by covering them with a plastic tarp cover lined with heavy-gage wire and secure to container edges with duct tape.
3) Place an opaque garbage bag overtop of each model in order to prevent photosynthesis.
4) Take one ambient air sample from the middle of the room by pumping a syringe several times.
5) Place the piece of tubing connected to the pump inside one of the holes drilled in the tarp piece. Place another piece of tubing inside the second hole, and attach a syringe.
6) Pump the syringe attached to the tubing of each model immediately after covering for a 0 minute gas sample. Remove the syringe and immediately replace with another.
7) Take samples of each model both 10 minutes and 30 minutes from when the first samples were taken.
8) Place samples in the fridge for up to 48 hours until they can be brought to the lab for analysis.
Taking Gas Sample |
Airtight Chamber |
Gas Chromatograph |