|
|
|
NOTE: It is not very crucial to read all of the context, though it would aid in understanding the reaction that is used in my investigation. It would be recommended to at least read the abstract before going reading the investigation. 
Name: Donna Lee School: Sisler High School Grade: 10-12 Team size: 1 Subject Area: Biology/chemistry Project Type: experimental Language: English Software: Flash 8, Dreamweaver, FileZilla, Notepad, Adobe Photoshop Hardware:digital camera Idea Source: When I was working with a virologist at the Microbiology lab, I was following under his guidance, which was mainly on PCR (polymerase chain reaction) - it was sometime later in April that I wondered how the PCR would be affected by changing one of the reaction reagents. Skills: HTML, how to use Flash, Photoshop, organization Awards: none :( Summary:
To express in very general terms, the focus of my project involves a reaction called PCR (polymerase chain reaction – further explained in context section). PCR is a reaction that reproduces vast quantities of DNA. I will specifically be using reverse-transcription real-time PCR (real-time PCR involves sophisticated equipment to detect product , reverse transcription is a step that converts RNA to DNA) to produce copies of a segment of the influenza virus (the matrix gene).
I want to change the concentration or optimize the magnesium chloride concentration so that the PCR could produce products with efficiency.
|