Methodology

 Project Information

 Abstract

 Project Summary

 Introduction

 Purpose/Hypothesis

 Materials

 Methodology

 Methodology Diagram

 Data

 Graphed Results

 Results Summarized

 Pictures

 Sources of Error

 Discussion

 Conclusion

 Applications

 Glossary

 References

 Acknowledgements

 Journal

1.      Fifteen Petri dishes were lined with filter paper, and placed on a table, in a room at
  22.8 degrees Celsius.

2.      The Petri dishes were divided into five groups of three. It was important to have
  large sample sizes, in order to produce accurate results.

3.      Each group of three Petri dishes was labeled accordingly to its content.

4.      The first group – Three Petri dishes were saturated with 5 ml of water each.
  (15 ml in total)

5.      The second group – Three Petri dishes were saturated with 5 ml of 3% hydrogen
  peroxide each. (15 ml in total)

6.      The third group – Three Petri dishes were each saturated with the content of a
  capsule filled with vitamin A palmitate (10 000IU). The capsules consisted of a
  clear gelatin envelope that was pricked with a pin in order to release its vitamin A  
  content.

7.      The fourth group – Three Petri dishes were each saturated with the content of a
  capsule filled with vitamin E d-alpha tocopheryl acetate (400IU). The capsules
  consisted of a clear gelatin envelope that was pricked with a pin in order to
  release the vitamin E content.

8.      The last group – Three Petri dishes were saturated with vitamin C. One
  effervescent ascorbic acid tablet was dissolved in 50ml of tap water. Then, 5ml of
  that solution was placed in each of the three Petri dishes. This was done to obtain
  a dose of vitamin C equivalent to the Recommended Daily Allowance.

9.      Fifty radish seeds were placed into each Petri dish. The number of seeds was
  chosen arbitrarily. It is important to have a large sample size for an experiment, to
  minimize bias in the results.

10.   Each Petri dish was then saturated with five ml of hydrogen peroxide.

11.   All of the groups were placed under a growing light. The distance between the
  growing light and the Petri dishes was 28cm.

12.  The germination of the seeds was recorded. A seed was considered germinated
  when it sprouted a root. Observations were recorded after about 3 days.

13.   Using radish seeds, the entire experiment was repeated 3 more times. The exact
  same method was used each time.

14.  The entire experiment was again repeated 4 times. However, this time, at step 10,
  fifteen L’Orto di Larosa yellow flat bean seeds were used instead of the radish
  seeds.

 

Back to the Canada Wide Virtual Science Fair

 Back to Home Page – Aaron Hakim