Analysis


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Among the three household cleaners, the laundry detergent Ariel has the highest degree of alkalinity compared to the shampoo Fructis and the dishwashing liquid Quix. According to the scale, it has a pH of 8 which makes it a weak base, and not an almost neutral liquid like the other two products tested. This fact can be associated with the laundry detergent's use. Since it has to dissolve difficult greasy stains and is not in a direct contact with our skin, it can contain much stronger chemicals.

The presence of a non - ionic surfactant, Alkyl Ethoxylate Sulfate, in Ariel's composition enabled me to obtain the most DNA strands from a sample of kiwi fruit. During three trials, an average of 0.73g of deoxyribonucleic acid was extracted with a minimum of 0.7g and a maximum of 0.8g (from an initial sample of 30g).

 

  Grease and soap

           (49) Removal of grease by the detergent

 

   Cell + detergent

    (49) Disruption of the cell membrane by the detergent

 

 

The least efficient detergent that was used during the experiment, was the shampoo Fructis. It  was composed of a weak surfactant - Sodium Lauryl Sulfate - thus it enabled me to extract an average of 0.33g of DNA strands, with a minimum of 0.30g and a maximum of 0.40g.

 

The chilean dishwashing liquid, Quix, can liberate from 0.40g up to 0.60g of deoxyribonucleic acid, with a three trial average of 0.5g. Composed of Alkyl Polyglycoside and N,N'-bis(3-aminopropyl) Propane-1,3-Diamine it precipitated more DNA than Fructis, but less than Ariel.

 

Alkyl Ethoxylate Sulfate contained in the laundry detergent Ariel, was able to bind easily with the lipids and the proteins that make up the cell membrane, therefore it liberated a large quantity of DNA.

 

The weaker chemicals used to fabricate the shampoo Fructis and the laundry detergent Quix were less efficient to disrupt the cell membrane. As a result, the amount of DNA liberated and then precipitated was smaller.


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The Best Detergent For Plentiful DNA Extraction
By Kevin Bachor kevin.bachor@gmail.com
Cirque du Soleil - Ecole Nationale de Cirque