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The experiment was performed in order to compare the efficiency of three
different household detergents during a DNA extraction process from a sample of
kiwi cells. Although relatively easy and requiring few advanced devices, the
method follows all three steps that are used during DNA extraction in a
professional laboratory: disruption of the cell membrane, liberation of the DNA,
and precipitation of the DNA. By finding a cheap, yet efficient, way of
extracting DNA strands we can reduce the costs related to buying expensive
chemicals by schools that perform this experiment for education
purposes. The process requires the addition of a detergent and salt solution, to
an invariable sample of mashed kiwi cells in order to break down the protective
cell membrane made of phospholipids and proteins. Three distinct detergents were
used to prepare the solution: shampoo Fructis (Sodium Lauryl
Sulfate),
dishwashing liquid Quix (Alkyl Polyglycoside and N,N'-bis(3-Aminopropyl)Propane-1,3-Diamine), and laundry detergent
Ariel (Alkyl Ethoxylate Sulfate). It was possible to compare the efficiency of those
products by weighing dry DNA strands collected, and determining which had the
greatest mass. The laundry detergent, Ariel, was the most efficient
compared to the others; its composition and unique ability to dissolve lipid and
protein stains liberated the largest quantity of DNA - a three trial average of
0.73g.

(47) DNA strand
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