Abstract


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The experiment was performed in order to compare the efficiency of three different household detergents during a DNA extraction process from a sample of kiwi cells. Although relatively easy and requiring few advanced devices, the method follows all three steps that are used during DNA extraction in a professional laboratory: disruption of the cell membrane, liberation of the DNA, and precipitation of the DNA. By finding a cheap, yet efficient, way of extracting DNA strands we can reduce the costs related to buying expensive chemicals by schools that perform this experiment for education purposes.  The process requires the addition of a detergent and salt solution, to an invariable sample of mashed kiwi cells in order to break down the protective cell membrane made of phospholipids and proteins. Three distinct detergents were used to prepare the solution:  shampoo Fructis (Sodium Lauryl Sulfate), dishwashing liquid Quix (Alkyl Polyglycoside and N,N'-bis(3-Aminopropyl)Propane-1,3-Diamine), and laundry detergent Ariel (Alkyl Ethoxylate Sulfate). It was possible to compare the efficiency of those products by weighing dry DNA strands collected, and determining which had the greatest mass. The laundry detergent, Ariel, was the most efficient compared to the others; its composition and unique ability to dissolve lipid and protein stains liberated the largest quantity of DNA - a three trial average of 0.73g.

 

                                            

                                                      (47) DNA strand

 



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The Best Detergent For Plentiful DNA Extraction
By Kevin Bachor kevin.bachor@gmail.com
Cirque du Soleil - Ecole Nationale de Cirque