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& Hypothesis
Materials
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Germination
Experiment:
144 Brassica napus cv. Westar seeds were put into 6
Petri dishes (24 seeds/Petri dish) for each NaCl concentration (0, 2, 5, 8, 10,
and 20 g/L). This was repeated for the four transgenic lines. Salt (2, 5, 8, 10,
or 20 g) was weighed and poured into bottles where 1L of water was added for
each bottle. Using a pipette, 3mL of the corresponding salt concentration was
put into each Petri dish. The Petri dishes were then sealed with parafilm to
prevent water evaporation. After 6 days, the number of fully germinated seeds
was counted. Pictures were taken with a digital camera. All of the seeds were
then autoclaved before disposal. The
experiment was repeated twice and the results from both were used for the
analysis in the result section.
Vegetative
Growth Experiment:
21 Brassica napus cv. Westar seeds were planted in 7x7cm square pots.
After 5 days when most of the plants had germinated, 15 of the most similar
plants were chosen to water with the salt (3 for each NaCl concentration at 0,
2, 5, 10, or 20 g/L, see
Figure 1 also).
This was repeated for the four transgenic lines. On the first five days after
germination each plant was watered with 40mL of their corresponding salt
concentration but after that the plants were watered with their corresponding
solution once a week and tap water on the other days. After 3 weeks, pictures
about growing plants were taken. After 4.5 weeks, one plant from each line
treated with different salt concentrations was cut above the soil and the fresh
weight of those plants was recorded. The remaining plants were allowed to grow
to flowering (bagged to avoid the pollen mixing after flowering). Pictures of
the flowering plants were taken with a digital camera. The seeds of those plants
will be collected when they are ready. All the plants and soil used in the
experiment were autoclaved before disposal.
Variables
Vegetative
Growth Experiment: Germination Experiment:
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