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Given
more time, I would like to do the following experiments to get more
results for my project:
1. Since the seeds are the part of canola that are useful, the weight of
seeds between wild type and transgenic lines tested in this project should
also be compared (one plant from each line treated at each salt
concentration was allowed to grow for collecting seeds).
2.
Although all four transgenic lines tested in this project were transformed
with same gene and they can be thought as a repetition of the samples in a
way, the laboratory supervisor said that each transgenic line had some
unique features and only one line would be used in a breeding program to
develop a new variety of canola. So I would like to
do
more repeated trials for vegetative growth experiment to fine tune the
results.
3.
The gene used to transform all four lines was meant to develop better
growth under stressed conditions. If the best lines are also combined with
other genes involved in salt tolerance (eg, vacuolar pump gene
investigated in Reference 5-7), the plants may have even better resistance
to salt.
4. Since the salinity level and soil composition
are different from place
to place in the Prairie Provinces, testing the growth of the transgenic
lines used in this project in different soil samples across the provinces
could have more significance.
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