COMPLETE LISTING OF MATERIALS
* latex gloves; paper towels
*wheat cultivar seeds from 48 cultivars developed in Canada over the past 160 years from the PGRC collection
*growing containers and potting soil, stakes, measuring equipment, scale, envelopes, adequate greenhouse space with artificial lights for approximately 120 days
*-80C freezer; freeze dryer
*many (100's) Eppendorf tubes (1.5, 2.0, 2.5 ml); many glass beads
*graduated cylinders, Erlenmeyer flasks
*mixer mill, water bath, vortexer, centrifuge, microwave, convention oven, crushed ice & bucket
*Qiagen’s DNeasy Plant Mini Kit
*RNAse, sterile water
*Fluorescent Dye solution: 10 ml Quantification Buffer (Tris HCI, EDTA, NaCl) plus 10Fl Hoechst 33258 dye
*24 UBC primers
*Fluorometer and 96 well fluorometer plate
*Agarose powder; 50x TAE buffer (salt solution)
*5 horizontal electrophesis tanks; gel tray and combs, autoclave tape and loading dye, scale and DNA size markers
*ethidium bromide dye
*electrical power pack, digital camera and computer
* common statistical packages: SAS, Excel, UPGMA